Lactobacillus acidophilus Rosell-52
Rosell-52 is a very well-known strain of acidophilus. The strain is registered in the prestigious 'Collection National de Culture de Microorganisms (CNCM)' at the Institut Pasteur in France, and was named by leading probiotic laboratories, Institut Rosell, which manufactures the strain and has subjected it to extensive testing. Lactobacillus acidophilus Rosell-52 is very often researched along with Lactobacillus rhamnosus Rosell-11®, and in combination these strains are often known by the name ‘Lacidofil®'
Lactobacillus acidophilus Rosell-52, also known as Lactobacillus helveticus Rosell-52 is a gram positive, homofermentative, facultative anaerobe. This is typically a rod shaped, non-sporulating strain that tends to form pairs or short chains. (Cocconcelli, 1997). As a β-galactosidase positive strain, it acts like lactase in the digestive system, and therefore may help to digest lactose.
The strain has been extensively used in many areas of research and found to have a wide range of potential benefits for gastrointestinal health, including its anti-pathogenic effects against undesirable bacteria such asHelicobacter pylori and Clostridium difficile. In combination with Lactobacillus rhamnosus Rosell-11, it has been shown to be one of the best strains to use for Antibiotic-Associated Diarrhoea (AAD), with this probiotic combination also being shown to withstand the damaging effects of the antibiotic treatment. Rosell-52 has also been featured in studies to explore the effects of probiotics in areas of health outside the digestive system, including immune function and mental health issues. It’s also interesting to note that a number of clinical trials using this probiotic strain have been conducted in young children, demonstrating its safety at various stages of life.
Lactobacillus acidophilus Rosell-52 – Safety and Survival
Lactobacillus acidophilus Rosell-52 is found in food supplements, often in combination with other strains. Its ability to survive gastric challenges such as stomach acid and biliary salts have been tested in vivo by microbiology Institute Rosell Lallemand. Such tests have demonstrated that L. acidophilus Rosell-52 is resistant to gastric acidity, with survival rates being optimal at pH 3 and above. They have also shown that Lactobacillus acidophilus Rosell-52 is able to survive high concentrations of bile, reporting that this strain should reach the gut alive.
A gold standard trial by Nagulesapillai, V. et al, (2015), used quantitative PCR methods to detect and quantify specific strains in faecal samples taken from participants who had taken a combination of two strains, including L. acidophilus Rosell-52 alongside antibiotic treatment. Tests showed L. acidophilus Rosell-52 was detected in 80% of faecal samples, showing excellent gastric survival rates.
The safety of this food supplement has also been investigated. Graheg-Ogden, D. (2018) conducted a risk assessment of Lactobacillus acidophilus Rosell-52 as an ‘other substance’ according to the food supplement directive 2002/46/EC. The report was written based on previous risk assessments and articles on the strain, concluding ‘it is unlikely that L. helveticus Rosell-52 ND causes adverse health effects in the general healthy population with a mature gastro-intestinal tract’.
In 2017 Manzano, S. et al, conducted a multi-centre, randomised, double-blind, placebo-controlled trial looking at the safety and tolerance of 3 strains in healthy infants, including L. acidophilus Rosell-52. The study on 221 healthy, full term infants aged 3-12 months reported no impact on growth, no serious adverse effects were experienced and any minor adverse events which occurred were equal across all groups, meaning that all studied strains, including L. acidophilus Rosell-52 are safe and well tolerated by infants.
This supports a study by Stojkovic, A. et al, (2016), which also reported good tolerance in young children under 5 years of age.
The Stojkovic, A. et al (2016) study trialled a food supplement containing 3 strains, including L. acidophilus Rosell-52, on children under 5 years, which resulted in an improvement to the children’s wellbeing, particularly in relation to respiratory comfort.
Safety studies conducted in infants & children are particularly valuable as they present the safety and tolerability in what is considered a more vulnerable population, going further to support the strains safety.
Lactobacillus acidophilus Rosell-52 and Antibiotic Associated Diarrhoea (AAD) and Clostridium difficile Infection
Antibiotics are one of the most widely-used drugs in medicine today, often with life-saving effects. For them to be fully effective, it is necessary for patients to complete the course of medication; however, antibiotics may prompt a variety of unpleasant side effects which can be so debilitating that many patients are unable to complete the treatment. Uncompleted courses of antibiotics are believed to be a cause of ‘antibiotic resistance’, as if not fully eradicated, pathogenic bacteria may mutate and become resistant to the drugs. Finding a remedy to alleviate side effects and enable patients to complete their courses of medication is therefore very important, and so probiotics have been explored as one possible solution.
In a gold standard, randomised double-blind, placebo-controlled clinical trial using 146 healthy adults, Lactobacillus acidophilus Rosell-52 was tested alongside the Lactobacillus rhamnosus Rosell-11 strain to assess their combined potential against antibiotic-associated diarrhoea. All subjects were given a broad spectrum antibiotic for one week, and a treatment group was also given a probiotic supplement to take alongside the drugs, and then for a further seven days after antibiotic treatment had ceased. The control group were given a placebo. The results showed that the probiotic significantly shortened the duration of AAD symptoms (Evans et al 2016).
A second trial was conducted using 244 children aged 0-17 years, who were all suffering from either acute respiratory, urinary, or digestive infections requiring antibiotic treatment. The subjects were split into two groups, with 117 subjects being given antibiotics in combination with a supplement containing Lactobacillus acidophilus Rosell-52 and Lactobacillus rhamnosus Rosell-11, and the other 127 subjects being given antibiotic therapy alone. Again, the results showed a lower incidence and decreased duration of AAD in the probiotic group, with an additional benefit of decreased Clostridium difficile levels noted (Maydannik V.et al., 2010).
Numerous other multicentre, randomised, placebo-controlled, double-blind trials have tested the efficacy of this probiotic combination (Lactobacillus acidophilus Rosell-52 and Lactobacillus rhamnosus Rosell-11) for the prevention of AAD in adults. In a trial using 214 subjects who were already receiving antibiotic treatment for respiratory tract infections, the participants were randomised to receive either the probiotic or a placebo for 14 days. All subjects recorded bowel frequency and stool consistency daily. Results illustrated that members of the probiotic group had reduced bowel frequency compared to those in the placebo group (Song et al., 2010).
An earlier trial conducted in 2005 used 57 children who were taking antibiotics. The children were split into two groups, with one group being given the Lacidofil® supplement. The results indicated that 36.5% of those in the control group went on to develop antibiotic-associated diarrhoea, whereas only 7.4% of those in the probiotic group suffered this side effect (Ivanko O. & Radutnaya E., 2005).
Further relevant studies: Aryayev M. & Kononenko N. (2009),Dial S. et al., (2003), Doder R. et al., (2013), D’Souza A. L. et al., (2002), Gnaytenko O. et al., (2009), Jirapinyo, P. et al., (2002), Liskovich, V. et al., (2010), Marushko Y. & Shef G., (2007), Maydannik V. et al., (2008), Patsera M. et al., (2010).
Lactobacillus acidophilus Rosell-52 and Irritable Bowel Syndrome (IBS)
Irritable Bowel Syndrome (IBS) is an umbrella term for a variety of different digestive symptoms, including bloating, cramps, diarrhoea, and constipation. The underlying cause of the condition is not clear, and the term is usually applied generically to these symptoms by medical professionals once any serious medical causes have been ruled out. At this point, doctors may prescribe various medications to help manage the symptoms. Research is linking the condition with the health of the gut flora, however, and probiotic supplements are becoming an increasingly popular natural adjunct or alternative to conventional interventions, and most of the featured strains in this database have been researched for this purpose. As part of the Lacidofil® supplement, Lactobacillus acidophilus Rosell-52 has been tested to try and determine whether it may offer any benefits in this condition.
A pilot study did indicate that this probiotic supplement may help to alleviate the symptoms of IBS. The trial used 50 patients who had been diagnosed with, and had suffered from, chronic IBS for at least ten years. The patients continued with their conventional medication but added in a daily probiotic supplement containing Lactobacillus acidophilus Rosell-52 and Lactobacillus rhamnosus Rosell-11. They were then monitored for a variety of digestive outcomes including changes in regularity, stool consistency and abdominal pain and distension. After four months of using the probiotic, 88% of the patients showed an overall reduction in IBS-related symptoms (Benes, Z. et al., 2006).
A more recent prospective, double-blind, randomised, controlled clinical trial was conducted using 76 adult patients. The subjects had all been suffering from acute diarrhoea, which in most cases was non-infectious. The patients were divided into two groups: a treatment group in which members were given Lacidofil for seven days, and a control group given a placebo supplement. The study found that the probiotic supplement shortened the duration of diarrhoea, and a statistically significant improvement in stool consistency was recorded. A small improvement in symptoms of abdominal pain, nausea, vomiting, bloating, headache, fever and tenesmus) was also noted (Simadibrata et al., 2013).
Further relevant studies: Chayka V. et al., (2006), Firmesse, O. et al., (2005), Kocian, J. et al., 1994, Rampengan et al., 2010, Skorodumova et al., (2007), Ten Bruggencate, (2015), Tlaskal, P. et al., (1995), Tlaskalet al., (2005), Wasowska-Krolikoeska K. et al., (1997), Zvyagintzeva et al., (2008).
Lactobacillus acidophilus Rosell-52 and Stress, Anxiety and Mood
Due to the increasing prevalence of mental health issues in the world’s population, extensive research is being conducted to try and determine the underlying physiological causes. A role for the gut flora and probiotics has been suggested, prompting a growing interest in the ‘gut-brain’ connection. In this area of probiotic research, Lactobacillus acidophilus Rosell-52 has also been tested in combination with Bifidobacterium longum Rosell-175, in supplement known sometimes as Probio’ Stick.
In June 2017 a small pilot study of 10 participants, with diagnosed depressive disorders, aimed to evaluate the effects of Lactobacillus acidophilus Rosell-52 and Bifidobacterium longum Rosell-175 on depression symptoms, such as low mood, anhedonia (inability to feel pleasure) and sleep disturbance. Participants were assessed using the Montgomery-Asberg depression rating scale to monitor mood symtoms, and sleep was monitored by observing brain activity, heart rate, and eye movement during the night. Participants supplemented with probiotics showed significant improvement in their symptoms, including improved sleep quality, mood score, and the ability to feel pleasure (Wallace C, et al., 2017).
A randomised, double blind, placebo-controlled trial took 55 healthy participants and gave them a supplement containing either the above probiotic combination, or a placebo, for a period of thirty days. The results were measured using various validated scales of anxiety and stress as well as depression and ‘coping’ scales. The findings showed that the use of this combined probiotic significantly reduced the scores of anxiety, depression and stress, and could have beneficial psychological effects in humans. A pre-clinical study in which the probiotic supplement was administered to rats also indicated that the Lactobacillus acidophilus Rosell-52 and Bifidobacterium longum Rosell-175 combination had anxiolytic-like activity (anti-anxiety effect) (Messaoudi et al., 2011).
An earlier double-blind, placebo-controlled, randomised study was conducted on participants with symptoms of stress. The subjects received either the Probio’ Stick probiotic combination (37 participants) or a placebo (38 participants) for three weeks. It was found the probiotic supplement significantly reduced stress-induced gastrointestinal symptoms, such as abdominal pain and nausea/vomiting, in the treatment group (Diop et al., 2008).
Further relevant study: Yamamura S, et al. (2009)
Lactobacillus acidophilus Rosell-52 and Helicobacter pylori
Helicobacter pylori (H. pylori) is a species of bacteria that is often found residing in the human body, and it is thought to be present in around two thirds of the world's population. It typically lives in the digestive tract and, in most people, does not cause any noticeable symptoms; however, it can be a common cause of stomach ulcers.
It can be difficult to eradicate H. pylori once it becomes an issue, as it burrows into the stomach wall and 'hides'. The standard medical treatment from the doctor is a 'triple therapy' of two different antibiotics in combination with an antacid known as a proton pump inhibitor (PPI), but the treatment is not always completely successful. It is thought that probiotics may firstly help to reduce any negative side effects of the antibiotics, but may also have anti-pathogenic effects that could aid in eradicating this undesirable bacteria and make the treatment more effective.
A study was designed to evaluate the efficacy of the standard triple anti-H-pylori therapy both with, and without, a probiotic combination taken alongside the medication. To test this, 152 H. pylori-positive symptomatic patients were given the Lacidofil supplement alongside their 10 day triple therapy, and results were compared to a control group who had received the conventional treatment without a probiotic. Tests were conducted six weeks after therapy and it was found that those in the control group experienced a 72% success rate for eradication of the bacteria; however, in those subjects who had received the triple therapy plus the probiotic, the H. pylori eradication rate increased up to 92% (Bielanski et al., 2002).
Further relevant studies: Abaturov et al., (2014), Babak O., (2007), Gnaytenko et al., (2009), Plewinska E., (2006), Vdovychenko V., (2008), Ziemniak, W., (2006).
Lactobacillus acidophilus Rosell-52 and Immunity
As approximately 70% of the cells involved in our immune responses are located in our intestinal wall, the interaction between our resident microbiota and the immune system has been the subject of considerable scientific interest. Research has indicated that there is a significant interplay between the gut flora and the immune system, and a potential role for probiotics in regulating and enhancing immune function. Lactobacillus acidophilus Rosell-52 is one of the probiotic strains that has been featured in associated studies.
In a randomised, double-blind, placebo-controlled study which was performed on 135 children aged 3-7 years during the winter months, Lactobacillus acidophilus Rosell-52 was used as part of a probiotic supplement that also included the strains Bifidobacterium infantis Rosell-33, Bifidobacterium bifidum Rosell-71 and a prebiotic in the form of Fructooligosaccharides (FOS). It was found that this probiotic combination specifically reduced the incidence of gastric disorders, and ear-nose-throat (ENT) or bronchopulmonary infections by 25%, with an associated 40% reduction in number of days absent from school due to sickness (Cazzola, et al. 2010).
Further relevant studies: Cazzola, M. et al., (2010), Marschan E. et al (2008)
Lactobacillus acidophilus Rosell-52 and Lactose intolerance
Lactose intolerance is an increasingly common digestive issue, caused when the body is unable to digest the milk sugar lactose. As probiotic bacteria may help to break down lactose in the digestive system, their use to help alleviate this condition is being explored. The Lacidofil supplement is one of the probiotic combinations being researched for this purpose.
A small study using 19 patients with lactose intolerance attempted to demonstrate the efficacy of this probiotic supplement when given alongside dairy products such as milk, cottage cheese, and Edam cheese. Lactose tolerance and stool consistency were recorded both before and after the supplementation period of two weeks. The results indicated that Lactobacillus acidophilus Rosell-52 combined with Lactobacillus rhamnosus Rosell-11 helped to improve tolerance to the dairy foods by one third; this improvement in lactose tolerance was particularly significant when consuming milk (Kocian J., 1994).
Further relevant studies: Chernyshov, (2007), Chernyshov, (2009a). Chernyshov, (2009b) Kocian J., (1996).
Lactobacillus acidophilus Rosell-52 and Vaginal Dysbiosis
Vaginal dysbiosis (an imbalance of good & bad bacteria), typically presenting in the form of vaginal thrush, may be another unpleasant side effect of antibiotic treatment. Building on the outcome of previous research for antibiotic-associated diarrhoea, a 2010 study explored whether taking a probiotic supplement may also help to prevent vaginal symptoms in women taking antibiotics. A total of 96 women were used in the study; they had all undergone Caesarean section and were being given preventative antibiotics for seven days following the surgery. There were 56 women in the treatment group, all of whom were given a probiotic containing Lactobacillus acidophilus Rosell-52 and Lactobacillus rhamnosus Rosell-11 alongside their antibiotics, and a control group of 40 women who were given antibiotics only.
After the treatment, the integrity of the vaginal flora was seen to be preserved in 89.3% of the probiotic group, and no incidence of AAD was recorded. By comparison, in the control group a disruption in the vaginal flora was noted in all cases, and there was a 10% incidence of diarrhoea Liskovich et al., (2010).
Further relevant studies: Chayka et al., (2006).Also relevant: see all strains in the database which have been researched for vaginal health.
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